2019

Plant Journal

Rocznik: 2019 | Tom: 98 | Numer: 3

artykuł naukowy

angielski

EN Abscisic acid (ABA) integrates internal and external signals to coordinate plant development, growth and architecture. It plays a central role in stomatal closure, and prevents germination of freshly produced seeds and germination of non-dormant seeds under unfavorable circumstances. Here, we describe a Medicago truncatula ATP-binding cassette (ABC) transporter, MtABCG20, as an ABA exporter present in roots and germinating seeds. In seeds, MtABCG20 was found in the hypocotyl-radicle transition zone of the embryonic axis. Seeds of mtabcg20 plants were more sensitive to ABA upon germination, due to the fact that ABA translocation within mtabcg20 embryos was impaired. Additionally, the mtabcg20 produced fewer lateral roots and formed more nodules compared with wild-type plants in conditions mimicking drought stress. Heterologous expression in Arabidopsis thaliana provided evidence that MtABCG20 is a plasma membrane protein that is likely to form homodimers. Moreover, export of ABA from Nicotiana tabacum BY2 cells expressing MtABCG20 was faster than in the BY2 without MtABCG20. Our results have implications both in legume crop research and determination of the fundamental molecular processes involved in drought response and germination. Significance Statement In the presented manuscript we introduce the MtABCG20, a half-size ABC transporter of the G subfamily, as a modulator of abscisic acid distribution in model legume Medicago truncatula, participating in seed germination as well as root morphology and nodulation. Our results have implications both in legume crop research and determination of the fundamental molecular processes involved in drought response and germination.

511 - 523

10.1111/tpj.14234

http://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14234

CC BY-NC-SA (uznanie autorstwa - użycie niekomercyjne - na tych samych warunkach)

ostateczna wersja opublikowana

01.2019

przed opublikowaniem

140

140