2019

Phytochemistry Letters

Rocznik: 2019 | Tom: 30

artykuł naukowy

angielski

EN The aim of this study was to evaluate the utility of a hydroalcoholic extract of Hippocastani flos (HF) in wound healing, especially in regards to cell viability, proliferation, reactive oxygen species (ROS) generation and proinflammatory cytokine production using an endothelial cell line [human umbilical vein endothelial cell (HUVEC) line EAhy926]. Antibacterial and anti-hyaluronidase activity was also evaluated. The phytochemical profile was determined using an the UPLC-MS. The UPLC-MS analysis confirmed the presence of nine flavonoids, mainly kaempferol and quercetin derivatives. Specifically, the HF extract contained a high content of polyphenols (88.84 +/- 4.13 mg chlorogenic acid equivalents/g dw), including flavonoids (15.68 +/- 0.04 mg/quercetin equivalents/g dw). Antioxidant properties were demonstrated using FRAP assays (IC0.5= 159.82 mu g/mL). The HF extract exhibited a level of anti-hyaluronidase activity (IC50 = 0.86 mg/mL) similar to the positive control kaempferol (IC50 = 0.78 mg/mL). The antimicrobial properties of HF were shown against Staphylococcus aureus (MIC, MBC = 12.5 mg/mL). After exposing the endothelial cells to HF extract in vitro, it did not observed any change in the cell viability, proliferation, wound healing or proinflammatory cytokine production, but there was a dose dependent-reduction in ROS generation. In conclusion, HF extract exhibits anti-hyaluronidase activity, antimicrobial properties, and reduces ROS production. These observations may partly justify the use of HF in supporting wound healing.

367 - 375

10.1016/j.phytol.2019.02.031

https://www.sciencedirect.com/science/article/pii/S1874390018302404?via%3Dihub

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