Tytuł
Analysis of oral microbiome from fossil human remains revealed the significant differences in virulence factors of modern and ancient Tannerella forsythia
Autorzy
[ 1 ] Instytut Chemii Bioorganicznej Polskiej Akademii Nauk, Polska Akademia Nauk | [ P ] pracownik
Dyscyplina naukowa (Ustawa 2.0)
Rok publikacji
2020
Opublikowano w
Typ artykułu
artykuł naukowy
Język publikacji
angielski
Słowa kluczowe
Streszczenie
EN Background:Recent advances in the next-generation sequencing (NGS) allowed the metagenomic analyses ofDNA from many different environments and sources, including thousands of years old skeletal remains. It has beenshown that most of the DNA extracted from ancient samples is microbial. There are several reports demonstratingthat the considerable fraction of extracted DNA belonged to the bacteria accompanying the studied individualsbefore their death.Results:In this study we scanned 344 microbiomes from 1000- and 2000- year-old human teeth. The datasetsoriginated from our previous studies on human ancient DNA (aDNA) and on microbial DNA accompanying humanremains. We previously noticed that in many samples infection-related species have been identified, among themTannerella forsythia, one of the most prevalent oral human pathogens. Samples containing sufficient amount ofT.forsythiaaDNA for a complete genome assembly were selected for thorough analyses. We confirmed that theT.forsythia-containing samples have higher amounts of the periodontitis-associated species than the control samples.Despites, other pathogens-derived aDNA was found in the tested samples it was too fragmented and damaged toallow any reasonable reconstruction of these bacteria genomes. The anthropological examination of ancient skullsfrom which theT. forsythia-containing samples were obtained revealed the pathogenic alveolar bone loss in toothareas characteristic for advanced periodontitis. Finally, we analyzed the genetic material of ancientT. forsythiastrains. As a result, we assembled four ancientT. forsythiagenomes - one 2000- and three 1000- year-old. Theircomparison with contemporaryT. forsythiagenomes revealed a lower genetic diversity within the four ancientstrains than within contemporary strains.We also investigated the genes ofT. forsythiavirulence factors and foundthat several of them (KLIKK protease andbspAgenes) differ significantly between ancient and modern bacteria.
Strony (od-do)
402
Typ licencji
CC BY-ND (uznanie autorstwa - bez utworów zależnych)
Wersja tekstu w otwartym dostępie
ostateczna wersja opublikowana
Data udostępnienia
15.06.2020
Czas udostępnienia publikacji w sposób otwarty
w momencie opublikowania
Punktacja Ministerstwa / czasopismo
140
Punktacja Ministerstwa / czasopismo w ewaluacji 2017-2021
140
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