2020

Journal of Applied Genetics

Rocznik: 2020 | Tom: 61 | Numer: 4

artykuł naukowy

angielski

EN Lr46/Yr29/Pm39(Lr46) is a gene for slow rusting resistance in wheat. The aim of the study was to analyze the miRNA expression in selected common wheat cultivars carrying resistance genes,Lr46among others (HN Rod, Pavon'S', Myna'S', Frontana'S', and Sparrow'S') in response to leaf rust infection caused byPuccinia triticinaErikss. In the Pavon 'S', Myna 'S', Frontana'S', and Sparow'S' varieties a product with a length of 242 bp has been identified, which is specific to theXwmc44marker linked to the brown rust resistance geneLr46. In the next step, the differences in the expression of microRNA (miR5085 and miR164) associated with theLr46gene, which is responsible for different resistance of selected wheat cultivars to leaf rust, were examined using emulsion PCR (ddPCR). In the experiment, biotic stress was induced in mature plants by infecting them with fungal spores under controlled conditions in a growth chamber. For analysis the plant material was collected before inoculation and 6, 12, 24, and 48 h after inoculation. The experiments also showed that plant infection withPuccinia triticinaresulted in an increase in miR164 expression in cultivars carrying theLr46gene. The expression of miR164 remained stable in a control cultivar (HN ROD) lacking this gene. This has proved that miR164 can be involved in leaf rust resistance mechanisms.

503 - 511

10.1007/s13353-020-00573-5

http://link.springer.com/content/pdf/10.1007/s13353-020-00573-5.pdf

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ostateczna wersja opublikowana

08.2020

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