2020

Frontiers in Plant Science

Rocznik: 2020 | Tom: 11

artykuł naukowy

angielski

EN Glutamate dehydrogenase (GDH) releases ammonia in a reversible NAD(P)(+)-dependent oxidative deamination of glutamate that yields 2-oxoglutarate (2OG). In current perception, GDH contributes to Glu homeostasis and plays a significant role at the junction of carbon and nitrogen assimilation pathways. GDHs are members of a superfamily of ELFV (Glu/Leu/Phe/Val) amino acid dehydrogenases and are subdivided into three subclasses, based on coenzyme specificity: NAD(+)-specific, NAD(+)/NADP(+)dual-specific, and NADP(+)-specific. We determined in this work that the mitochondrialAtGDH1 isozyme fromA. thalianais NAD(+)-specific. Altogether,A. thalianaexpresses three GDH isozymes (AtGDH1-3) targeted to mitochondria, of whichAtGDH2 has an extra EF-hand motif and is stimulated by calcium. Our enzymatic assays ofAtGDH1 established that its sensitivity to calcium is negligible.In vivotheAtGDH1-3 enzymes form homo- and heterohexamers of varied composition. We solved the crystal structure of recombinantAtGDH1 in the apo-form and in complex with NAD(+)at 2.59 and 2.03 angstrom resolution, respectively. We demonstrate also that both in the apo form and in 1:1 complex with NAD(+), it formsD(3)-symmetric homohexamers. A subunit ofAtGDH1 consists of domain I, which is involved in hexamer formation and substrate binding, and of domain II which binds coenzyme. Most of the subunits in our crystal structures, including those in NAD(+)complex, are in open conformation, with domain II forming a large (albeit variable) angle with domain I. One of the subunits of theAtGDH1-NAD(+)hexamer contains a serendipitous 2OG molecule in the active site, causing a dramatic (similar to 25 degrees) closure of the domains. We provide convincing evidence that the N-terminal peptide preceding domain I is a mitochondrial targeting signal, with a predicted cleavage site for mitochondrial processing peptidase (MPP) at Leu17-Leu18 that is followed by an unexpected potassium coordination site (Ser27, Ile30). We also identified several MPD [(+/-)-2-methyl-2,4-pentanediol] binding sites with conserved sequence. AlthoughAtGDH1 is insensitive to MPD in our assays, the observation of druggable sites opens a potential for non-competitive herbicide design.

754

10.3389/fpls.2020.00754

http://dx.doi.org/10.3389/fpls.2020.00754

CC BY-NC-SA (uznanie autorstwa - użycie niekomercyjne - na tych samych warunkach)

ostateczna wersja opublikowana

06.2020

w momencie opublikowania

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