2020

RNA

Rocznik: 2020 | Tom: 26 | Numer: 12

artykuł naukowy

angielski

EN RNA G-quadruplexes fold almost exclusively into parallel-stranded structures and thus display much less structural diversity than their DNA counterparts. However, also among RNA G-quadruplexes peculiar structural elements can be found which are capable of reshaping the physico-chemical properties of the folded structure. A striking example is provided by a uridine tetrad (U-tetrad) placed on the 3'-terminus of the tetramolecular G-quadruplex. In this context, the U-tetrad adopts a unique conformation involving chain reversal and is responsible for a tremendous stabilization of the G-quadruplex (Delta T-m up to 30 degrees C). In this report, we attempt to rationalize the origin of this stabilizing effect by concurrent structural, thermal stability, and molecular dynamics studies of a series of G-quadruplexes with subtle chemical modifications at their 3'-termini. Our results provide detailed insights into the energetics of the "reversed" U-tetrad motif and the requirements for its formation. They point to the importance of the 2'OH to phosphate hydrogen bond and preferential stacking interactions for the formation propensity and stability of the motif.

2000 - 2016

10.1261/rna.076539.120

https://doi.org/10.1261/rna.076539.120

CC BY-ND (uznanie autorstwa - bez utworów zależnych)

ostateczna wersja opublikowana

05.2021

12 miesięcy po opublikowaniu

140

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