2020

Cells

Rocznik: 2020 | Tom: 9 | Numer: 9

artykuł naukowy

angielski

EN Circular RNAs (circRNAs) are the products of the non-canonical splicing of pre-mRNAs. In contrast to humans and animals, our knowledge of the biogenesis and function of circRNAs in plants is very scarce. To identify proteins involved in plant circRNA generation, we characterized the transcriptomes of 18Arabidopsis thalianaknockout mutants for genes related to splicing. The vast majority (>90%) of circRNAs were formed in more than one variant; only a small fraction of circRNAs was mutant-specific. Five times more circRNA types were identified incbp80and three times more inc2h2mutants than in the wild-type. We also discovered that incbp80,c2h2andflkmutants, the accumulation of circRNAs was significantly increased. The increased accumulation of circular transcripts was not accompanied by corresponding changes in the accumulation of linear transcripts. Our results indicate that one of the roles of CBP80, C2H2 and FLK in splicing is to ensure the proper order of the exons. In the absence of one of the above-mentioned factors, the process might be altered, leading to the production of circular transcripts. This suggests that the transition toward circRNA production can be triggered by factors sequestering these proteins. Consequently, the expression of linear transcripts might be regulated through circRNA production.

1937

10.3390/cells9091937

https://www.mdpi.com/2073-4409/9/9/1937/pdf

CC BY-ND (uznanie autorstwa - bez utworów zależnych)

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08.2020

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