2020

Nucleic Acids Research

Rocznik: 2020 | Tom: 48 | Numer: 6

artykuł naukowy

angielski

EN Three-dimensional structures have been solved for several naturally occurring RNA triple helices, although all are limited to six or fewer consecutive base triples, hindering accurate estimation of global and local structural parameters. We present an X-ray crystal structure of a right-handed, U.A-U-rich RNA triple helix with 11 continuous base triples. Due to helical unwinding, the RNA triple helix spans art average of 12 base triples per turn. The double helix portion of the RNA triple helix is more similar to both the helical and base step structural parameters of A'-RNA rather than A-RNA. Its most striking features are its wide and deep major groove, a smaller inclination angle and all three strands favoring a C3'-endo sugar pucker. Despite the presence of a third strand, the diameter of an RNA triple helix remains nearly identical to those of DNA and RNA double helices. Contrary to our previous modeling predictions, this structure demonstrates that an RNA triple helix is not limited in length to six consecutive base triples and that longer RNA triple helices may exist in nature. Our structure provides a starting point to establish structural parameters of the so-called 'ideal' RNA triple helix, analogous to A-RNA and B-DNA double helices.

3304 - 3314

10.1093/nar/gkz1222

http://academic.oup.com/nar/article-pdf/48/6/3304/33795381/gkz1222.pdf

CC BY-ND (uznanie autorstwa - bez utworów zależnych)

ostateczna wersja opublikowana

04.2020

w momencie opublikowania

200

200